My biol 207 practice exam questions are so funny! Too bad I have no idea how to answer it…
“You are studying the gene Bieber (Bie) in Drosophila. A Bie4 amorphic mutant, when homozygous, dies at the pupal stage of development (pupae don’t make it to adulthood). You want to test the hypothesis that adding a normal Bie+ gene will rescue the Bie4 (amorph) phenotype in flies (ie. If it will let them live). You intend to do this by using a Bie+ transgene and testing for complementation, since you already have the Bie+ gene sequence cloned.
You use a transposon containing the full length Bieber gene (Bie+ ) to try and rescue the amorphic phenotype (death at the pupal stage).
Several transformed lines of Bie+ were obtained, called L1- L4.
L1 & L2 were able to rescue.”